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A novel procedure for generating both nitric oxide and superoxide in situ from chemical sources at any chosen mole ratio. First application: Tyrosine oxidation and a comparison with preformed peroxynitrite

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1021/tx0001272
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada
FormatTexte, Article
Sujetfree radical; nitric oxide; peroxynitrite; superoxide; tyrosine derivative; chemical reaction kinetics; oxidation; reaction analysis; synthesis; molsidomine; nitrates; nitric Oxide; nitric Oxide Donors; nitrogen Oxides; oxidants; oxidation-Reduction; spermine; superoxides; tyrosine
Résumé
The first method for generating ·NO and O2·- at any known, constant ratio has been developed. Spermine NONOate and di(4-carboxybenzyl)hyponitrite decay with first-order kinetics and exactly equal rate constants (half-lives of 80 min) at 37 °C and pH 7.5 to give 200 and 40 mol % ·NO and O2·- respectively. Tyrosine oxidation to dityrosine and 3-nitrotyrosine (the major and minor products under the conditions used in these experiments) has been studied (mainly in the presence of CO2) using various different ratios of the rates of formation of ·NO and O2·-. The ·NO/O2·- = 1.0 product profiles are very similar to those of the products derived from equal amounts of ·NO and O2·- generated at a ·NO/O2·- ratio of 1.0 from SIN-1 but are very different from those derived from preformed peroxynitrite. All the experimental results can be explained in terms of free radical chemistry. The product profiles at all the ·NO/O2·- ratios could be satisfactorily simulated provided an important group of reactions which lead to the consumption of dityrosine was included.
Date de publication
Dans
  • Chemical Research in Toxicology 13, nº 12 (2000) : 1287–1293.
Langueanglais
Publications évaluées par des pairsOui
Numéro NPARC21276745
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Identificateur de l’enregistrement7d75aad7-7883-4557-a26c-2c201260f8b8
Enregistrement créé2015-10-13
Enregistrement modifié2020-03-26
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