| DOI | Trouver le DOI : https://doi.org/10.1007/BF02374831 |
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| Auteur | Rechercher : Nelson, Louise M.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
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| Format | Texte, Article |
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| Sujet | acetylene reduction; ammonium nitrate; isolate variability; leghemoglobin; pea; Pisum sativum; Rhizobium leguminosarum |
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| Résumé | This study was conducted to determine the effect of short term application of NH₄NO₃ on nodule function and to determine whether the rhizobial isolate used was a significant factor in this effect. Pea plants were inoculated with 10 differentRhizobium leguminosarum isolates and grown for 3 weeks in N-free medium before addition of 0, 1, 2 or 5 mM NH₄NO₃ for 2 to 7 days. Acetylene reduction and leghemoglobin content decreased with increasing exposure time to NH₄NO₃ and with increasing concentration of NH4NO3. NH₄⁺ and NO₃⁻ depletion from the nutrient medium were assayed in plants exposed to 5 mM NH₄NO₃ and mean uptake rates were similar for each ion. There were significant differences among isolates in the rate of decrease of C₂H₂ reduction with increasing NH₄NO₃ concentration (C₂H₂ reduction responsiveness to NH₄NO₃) 4 and 7 days after addition of NH₄NO₃ but no differences after 2 days of exposure to NH₄NO₃. There were significant differences among isolates in NH₄⁺ depletion from the nutrient medium but these differences were not correlated with the differences observed in C₂H₂ reduction. Ranking of the isolates for C₂H₂ reduction responsiveness to NH₄NO₃ applied to plants with nodules was different from that obtained when NH₄NO₃ was applied at seeding. Isolates with varying sensitivity to NH₄NO₃ may be useful tools for determining the mechanisms responsible for inhibition of symbiotic N₂ fixation by combined nitrogen. |
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| Date de publication | 1987-06 |
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| Maison d’édition | Springer Nature |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | BF02374831 |
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| Numéro du CNRC | NRCC-25863 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 7b72443d-b906-4536-8090-f1bddfd9c30e |
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| Enregistrement créé | 2023-06-29 |
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| Enregistrement modifié | 2023-07-03 |
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