| DOI | Trouver le DOI : https://doi.org/10.1007/978-3-642-18713-1_1 |
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| Auteur | Rechercher : Macmanus, J. P.1; Rechercher : Jian, M.1; Rechercher : Preston, E.1; Rechercher : Webster, J.1; Rechercher : Zurakowski, B.1 |
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| Affiliation | - Conseil national de recherches Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Chapitre de livre |
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| Sujet | E2F1 transcription factor; focal ischemia; infarct-apoptosis; microtubule; microtubule-associated protein 2 |
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| Résumé | Since cultured neurons which are deficient in the transcription factor E2F1 display a resistance to a wide variety of insults, studies were undertaken to see if this resistance extended to a cerebral ischemic insult. Following 2 h of left middle cerebral artery occlusion and 1 d of reperfusion a 33% smaller infarct (p <0.05) was observed by 2,3,5 triphenyltetrazolium staining in the brains of E2F1-null animals compared to E2F1 and littermate mice. No differences in physiological parameters or cerebrovasculature were observed in the three groups of animals. A milder ischemic insult produced by 20 min of MCA occlusion and 7 d of reperfusion produced a greater difference in the E2F1-null animals with a 71% smaller infarct (p<0.001) compared to littermate controls. A decrease in ischemic injury in E2F1-null mice was also observed by immunohistochemical monitoring of the neuronal-specific MAP2 cytoskeletal protein and TUNEL staining. Finally a battery of six tests of motor function showed both E2F1 and -/littermates were similarly impaired at 1 d of reperfusion, but by 7 d the E2F1 -/mice improved significantly better compared to the wild type mice (p < 0.01) and ended up only slightly impaired by that time. It is concluded that the transcription factor E2F1 does modulate neuronal viability in brain after cerebral ischemia. |
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| Date de publication | 2004 |
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| Maison d’édition | Springer |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | MACMANUS2002 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 7829b579-a87e-467d-b25e-ce989cb919e5 |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2020-06-12 |
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