Identification of the catalytic nucleophile in the cellulase from Schizophyllum commune and assignment of the enzyme to Family 5, subtype 5 of the glycosidases

DOI	Trouver le DOI : https://doi.org/10.1016/S0014-5793(97)01049-1
Auteur	Rechercher : Clarke, Anthony J.; Rechercher : Drummelsmith, Jolyne; Rechercher : Yaguchi, Makoto¹
Affiliation	Conseil national de recherches Canada. Institut des sciences biologiques du CNRC
Format	Texte, Article
Résumé	Differential chemical modification of the cellulase from Schizophyllum commune with [N-methyl-3H]1-ethyl-3(4-azonia-4,4-dimethylpentyl)-carbodiimide in the presence and absence of substrate identified an active site glutamate residue within the peptide: Leu-Gln-Ala-Ala-Thr-Glu-Trp-Leu-(Lys). This Glu residue is proposed to participate in binding of substrate as amino acid sequence homology studies combined with mechanism-based inhibition of the cellulase with 4',5'-epoxypentyl-beta-D-cellobioside identified a neighboring Glu residue, which conforms to the Glu-X-Gly motif of Family 5 glycosidases, as the catalytic nucleophile. These data allow the assignment of the S. commune cellulase to Family 5, subtype 5 of the glycosidases.
Date de publication	1997-09-08
Dans	FEBS Letters 414, nº 2 (8 septembre 1997) : 359–361.
Langue	anglais
Numéro du CNRC	CLARKE1997
Numéro NPARC	9383971
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	766c1791-0c5c-4c54-bc78-32cdd6d0130c
Enregistrement créé	2009-07-10
Enregistrement modifié	2020-03-20

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