| Téléchargement | - Voir le manuscrit accepté : Detection of azaspiracids in mussels using electrochemical immunosensors for fast screening in monitoring programs (PDF, 5.0 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1016/j.snb.2018.02.046 |
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| Auteur | Rechercher : Leonardo, Sandra; Rechercher : Kilcoyne, Jane; Rechercher : Samdal, Ingunn A.; Rechercher : Miles, Christopher O.1; Rechercher : O’sullivan, Ciara K.; Rechercher : Diogène, Jorge; Rechercher : Campàs, Mònica |
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| Affiliation | - Conseil national de recherches Canada. Science des mesures et étalons
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| Format | Texte, Article |
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| Sujet | azaspiracid; antibody; electrochemical immunosensor; protein G; biotin; mussel |
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| Résumé | Given the widespread occurrence of azaspiracids (AZAs), it is clearly necessary to advance in simple and low-cost methods for the rapid detection of these marine toxins in order to protect seafood consumers. To address this need, electrochemical immunosensors for the detection of AZAs based on a competitive direct immunoassay using peroxidase-labelled AZA as a tracer were developed. An anti-AZA polyclonal antibody was immobilised in a controlled and stable manner on protein G or avidin-coated electrodes. Experimental conditions were first optimised using colorimetric immunoassays on microtitre plates, providing intermediate products already applicable to the accurate detection of AZAs. Then, transfer of the protein G and avidin–biotin interaction-based immunoassays to 8-electrode arrays provided compact and miniaturised devices for the high-throughput detection of AZAs. The low amounts of immunoreagents required as well as the potential for reusability of the avidin–biotin interaction-based immunosensors represented significant economic savings as well as a contribution to sustainability. The electrochemical immunosensors enabled the quantification of all regulated AZAs below the regulatory limit, as well as a broad range of other toxic AZA analogues (from 63 ± 3 to 2841 ± 247 μg AZA-1 equiv./kg for the protein G-based immunosensor and from 46 ± 2 to 3079 ± 358 μg AZA-1 equiv./kg for the avidin–biotin interaction-based immunosensor). The good agreement between the results obtained by the immunosensors and LC–MS/MS in the analysis of naturally contaminated mussel samples demonstrated the easy implementation of electrochemical immunosensors for routine analysis of AZAs in food safety monitoring programs. |
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| Date de publication | 2018-02-06 |
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| Maison d’édition | Elsevier |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23003585 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 71b3d66c-81a1-4d44-bfc5-74b842e4d41e |
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| Enregistrement créé | 2018-07-20 |
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| Enregistrement modifié | 2020-06-03 |
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