| DOI | Trouver le DOI : https://doi.org/10.1007/BF00274010 |
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| Auteur | Rechercher : Herzog, Albert; Rechercher : Yaguchi, Makoto1; Rechercher : Cabezon, Teresa; Rechercher : Corchuelo, Maria-Clara; Rechercher : Petre, Jean; Rechercher : Bollen, Alex |
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| Affiliation | - Conseil national de recherches Canada
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| Format | Texte, Article |
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| Sujet | gene cluster; phenylalanine; missense mutation; ribosomal protein; Escherichia coli |
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| Résumé | The conditionally lethal mutation, 286lmis, has been mapped inside the ribosomal protein gene cluster at 72 minutes on the Escherichia coli chromosome and was found to cotransduce at 97% with rpsE (S5). The 2861mis mutation leads to thermosensitivity and impaired assembly in vivo of 30S ribosomal particles at 42°C. The strain carrying the mutation has an altered S17 ribosomal protein; the mutational alteration involves a replacement of serine by phenylalanine in protein S17. Spontaneous reversion to temperature independence can restore the normal assembly in vivo of 30S ribosomal subunits at 42°C and the normal chromatographical sehaviour of the S17 ribosomal protein in vitro. We conclude therefore that the 2861mis mutation affects the structural gene for protein S17 (rpsQ). |
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| Date de publication | 1979-01 |
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| Maison d’édition | Springer |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | HERZOG1979 |
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| Numéro NPARC | 9376282 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 6c756b85-eaca-416d-a473-1785a2dfbbf6 |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2022-11-18 |
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