| Téléchargement | - Voir la version de l’auteur : A FAD2 homologue from Lesquerella lindheimeri has predominantly fatty acid hydroxylase activity (PDF, 5.8 Mio)
|
|---|
| DOI | Trouver le DOI : https://doi.org/10.1016/j.plantsci.2007.03.015 |
|---|
| Auteur | Rechercher : Dauk, Melanie; Rechercher : Lam, Patricia1; Rechercher : Kunst, Ljerk1; Rechercher : Smith, Mark A. |
|---|
| Affiliation du nom | - Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
|
|---|
| Format | Texte, Article |
|---|
| Sujet | lesquerella lindheimeri; fatty acid hydroxylase; ricinoleic acid; hydroxy fatty acid; seed oil content; single seed TLC |
|---|
| Résumé | A genomic sequence encoding a polypeptide with 91% sequence identity to the Lesquerella fendleri bifunctional oleate 12-hydroxylase:desaturase was amplified by PCR from Lesquerella lindheimeri. Expression of the gene in the yeast Saccharomyces cerevisiae resulted in the synthesis of ricinoleic acid and very low levels of di-unsaturated fatty acids. Comparison of the amino acid sequences of the L. lindheimeri and castor bean oleate 12-hydroxylase enzymes to those of the L. fendleri bifunctional oleate 12-hydroxylase:desaturase and oleate 12-desaturase enzymes from 32 diverse species identified a single amino acid (M295) that was conserved in the hydroxylases and different but also conserved in the desaturases and the bifunctional enzyme. Site-directed mutagenesis indicated that this residue was most likely not involved in determining the catalytic outcome of the hydroxylation/desaturation reaction. Transformation of an Arabidopsis fad2/fae1 mutant line with the L. lindheimeri hydroxylase gave further evidence that this enzyme, like the castor oleate 12-hydroxylase, is primarily a fatty acid hydroxylase and should not be considered bifunctional. Total hydroxy fatty acid content of up to 18% of seed fatty acids was measured in homozygous transformants. Lines with the highest hydroxy fatty acid content showed significant reduction in total oil content. |
|---|
| Date de publication | 2007-04-04 |
|---|
| Maison d’édition | Elsevier |
|---|
| Dans | |
|---|
| Langue | anglais |
|---|
| Publications évaluées par des pairs | Oui |
|---|
| Numéro NPARC | 23002566 |
|---|
| Exporter la notice | Exporter en format RIS |
|---|
| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
|---|
| Identificateur de l’enregistrement | 6c69af17-0d37-475c-8c4f-2d34d1cde352 |
|---|
| Enregistrement créé | 2017-11-30 |
|---|
| Enregistrement modifié | 2020-05-10 |
|---|
