| Auteur | Rechercher : Yan, F.; Rechercher : Gilbert, M.; Rechercher : Wakarchuk, Warren; Rechercher : Brisson, J.; Rechercher : Whitfield, Dennis |
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| Format | Texte, Article |
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| Sujet | Canada; Carbohydrate Conformation; chemical synthesis; chemistry; donor; ENZYME; enzymes; Glycosylation; Glycosyltransferases; LACTOSE; LINKAGES; metabolism; MOLECULE; NOE; Nuclear Magnetic Resonance,Biomolecular; oligosaccharide; Oligosaccharides; Polyethylene Glycols; Protein Binding; SHIGA-LIKE TOXIN; Shiga-Like Toxin I; Support,Non-U.S.Gov't; Synthesis; synthetic; transfer; TRISACCHARIDE; Trisaccharides |
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| Résumé | [reaction: see text]. A trisaccharide donor containing a cis-Galpalpha(1-->4)Galp linkage was prepared using a synthetic strategy based on chemoenzymatic oligosaccharide synthesis on a soluble polymeric support. Significantly, only retaining glycosyltransferases gave complete reactions, whereas inverting enzymes showed little or no activity with poly(ethylene glycol) (MPEG)-bound lactose as an acceptor. The MPEG-attached trisaccharide was shown to bind to Verotoxin-1 by transfer NOE studies through the Galpalpha(1-->4)Galp portion of the molecule |
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| Date de publication | 2001-10-18 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | YAN2001A |
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| Numéro NPARC | 9360321 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 657615ac-cdaf-47a5-874d-3cd65e103d1e |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2020-03-27 |
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