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Alterations in cell-cell communication in human papillomavirus type 16 (HPV16) transformed rat myoblasts

Par Conseil national de recherches du Canada

AuteurRechercher : 1; Rechercher : 2; Rechercher : 1; Rechercher : 3; Rechercher : ; Rechercher : ; Rechercher : 1; Rechercher : ; Rechercher : 1; Rechercher : 3
Affiliation
  1. Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
  2. Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
  3. Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
FormatTexte, Article
Sujetenvironmental
Résumé
A reduction of gap-junctional intercellular communication (GJIC) often accompanies neoplastic transformation. The present work demonstrates that transformation by the oncogenic human DNA virus, human papilloma virus 16(HPV16), also reduces GJIC between L6 rat myoblasts. HPVs are associated with anogenital cancers, the incidence of which is increasing in HIV positive patients of both sexes. Using videofluorescence imaging of Fura-2 loaded cells a lack of GJIC between transformed HPV16-L6 cells was first indicated by uncoordinated brief [Ca2+]i spikes in clusters of DMSO-treated HPV16-L6 cells instead of the synchronous, sustained [Ca2+]i surges in clusters of DMSO-treated L6 cells. Reduced GJIC between HPV16-L6 cells was demonstrated directly by a much reduced transfer of lucifer yellow dye from HPV16-L6 cells, which had been loaded with the dye through electroporation with an EPIZAP II in situ electroporator, to neighbouring nonelectroporated HPV16-L6 cells. One reason for this reduced GJIC between HPV16-L6 cells could have been their dramatically enhanced activity of membrane-associated PKC which is known to phosphorylate connexins and down-regulate gap junctions. However, the main reason was the viral-induced inhibition of the expression of a major gap junction component, Cx43 (Connexin 43), in the transformed myoblasts.
Date de publication
Dans
  • Cellular and Molecular Biology 41, nº 4 (1995) : 481–498.
Langueanglais
Numéro du CNRCENNAJI1995
34581
Numéro NPARC3539744
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Identificateur de l’enregistrement5c4feb33-dd33-4331-b08e-e54b06d3c348
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-29
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