| Résumé | Offline coupling of high performance liquid chromatography (HPLC) to dried droplet laser ablation (LA) ICP-MS detection is described. The method is based on the offline spotting of HPLC fractions onto a substrate followed by LA introduction of the dried sample residues to an ICP-MS for elemental analysis. Quantitation of selenomethionine (SeMet) in yeast using species specific isotope dilution (ID) was achieved following digestion of samples in 4 M methanesulfonic acid and HPLC separation of species. Chromatographic fractions having retention times of 180–210 seconds were collected for each sample. Dried micro-droplets from each fraction were ablated from a polystyrene substrate and quantitated for SeMet. Concentrations of 3301 ± 18 and 3309 ± 24 μg g−1 (one standard deviation, n = 4) with RSDs of 0.55% and 0.73% were obtained based on measured 78Se/74Se and 82Se/74Se ratios, in good agreement with the values of 3309 ± 19 and 3305 ± 26 μg g−1 (one standard deviation, n = 4, RSDs of 0.58% and 0.79%), respectively, obtained by direct HPLC-ICP-MS analysis. The proposed method provides a satisfactory alternative technique for the quantitation of SeMet in yeast. |
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