| DOI | Trouver le DOI : https://doi.org/10.1117/12.969611 |
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| Auteur | Rechercher : Mantsch, H. H.1; Rechercher : Surewicz, W. K.1; Rechercher : Muga, A.1; Rechercher : Moffatt, D. J.1; Rechercher : Casal, H. L.1 |
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| Affiliation | - Conseil national de recherches du Canada
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| Format | Texte, Chapitre de livre |
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| Conférence | 7th International Conference on Fourier Transform Spectroscopy, June 19, 1989, Fairfax, Virginia, USA |
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| Résumé | Infrared spectroscopy is being used increasingly to study the conformational structure of proteins and polypeptides in aqueous solution. The methodology generally used for the infrared spectroscopic analysis of protein secondary structure is based on three steps: 1. Separation of the overlapping amide I (amide C = O stretching) component bands via band-narrowing procedures such as Fourier self-deconvolution or derivation. 2. Assignment of the resolved component bands, based on previously established spectra-structure correlations, to different secondary structure elements, i.e., alpha helices, beta-sheets, turns or non-ordered conformations. 3. Extraction of quantitative information on protein secondary structure from analysis of amide I band profiles by curve fitting. Each of these three steps has potential sources of error which have to be recognized to prevent fallacious interpretations. |
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| Date de publication | 1989-12-01 |
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| Dans | |
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| Série | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23001099 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 41f5648a-fc75-4cb5-b271-baa4fb547441 |
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| Enregistrement créé | 2016-12-13 |
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| Enregistrement modifié | 2020-06-10 |
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