| DOI | Trouver le DOI : https://doi.org/10.1021/ac049318qS0003-2700(04)09318-7 |
|---|
| Auteur | Rechercher : Kanda, V.; Rechercher : Kariuki, J.; Rechercher : Harrison, D1; Rechercher : McDermott, Mark1 |
|---|
| Affiliation | - Conseil national de recherches Canada. Institut national de nanotechnologie
|
|---|
| Format | Texte, Article |
|---|
| Résumé | A simple method is presented for patterning of protein antigens at a gold surface for use in surface plasmon resonance (SPR) imaging experiments. Microfluidic devices fabricated from poly(dimethylsiloxane) were used to flow various fluids over a gold substrate in spatially defined channels. This technique was used to pattern the surface chemistry of the gold as well as to adsorb antigens from solution to the modified substrates. The resulting antigen arrays were probed with complementary antibodies in order to demonstrate the effectiveness of the patterning for antibody capture experiments. SPR imaging was used to aid in the optimization of array fabrication and to observe the interactions of unlabeled antibodies with these microarrays. This work presents a means of fabricating microarrays with controlled surface density of antigens. SPR imaging provides both quantitative and qualitative evaluation of antibody binding in a label free format. |
|---|
| Date de publication | 2004-12-15 |
|---|
| Dans | |
|---|
| Numéro NPARC | 12327947 |
|---|
| Exporter la notice | Exporter en format RIS |
|---|
| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
|---|
| Identificateur de l’enregistrement | 354e3038-d9c8-4e5a-ad27-1f24fe533db2 |
|---|
| Enregistrement créé | 2009-09-10 |
|---|
| Enregistrement modifié | 2020-04-17 |
|---|
