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Population differences in nerve resistance to paralytic shellfish toxins in softshell clam, Mya arenaria, associated with sodium channel mutations

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/s00227-006-0432-z
AuteurRechercher : ; Rechercher : 1; Rechercher : ; Rechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
FormatTexte, Article
Sujetmutation; nerves; nucleotide sequence; saxitoxin; sodium channels; sodium channels (voltage-gated); toxins; genomics; mRNA; Alexandrium bivalvia; Mya arenaria
Résumé
The softshell clam, Mya arenaria, is a commercially important bivalve with wide latitudinal distribution in North America. Populations of clams with a history of repeated exposure to toxic Alexandrium spp. have developed a natural resistance to the paralytic shellfish toxins (PSTs) produced by these algae. An association between PST resistance in individual clams and a single mutation in the saxitoxin (STX) binding region of the -subunit of the voltage-gated sodium (Na+) channel gene was previously identifed. Here we establish that more than one mutation associated with nerve resistance to STX occurred at this locus. Both cDNA from mRNA and genomic DNA sequences from individual clams are identical demonstrating that both alleles are expressed simultaneously. In addition, one resistant allele per individual is suYcient to confer neural resistance to STX even though heterozygous individuals show an intermediate level of resistance to STX in in vitro nerve trunk assays.
Date de publication
Conditions d’utilisation
  • © Springer-Verlag 2006
Dans
  • Marine Biology 150, nº 6 (15 septembre 2006) : 1227–1236.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRC1223
NRCC 55853
Numéro NPARC3538035
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Identificateur de l’enregistrement343e0527-1ee7-45a3-a5b6-a1c11075e4de
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-22
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