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Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems

DOITrouver le DOI : https://doi.org/10.1039/b409366f
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Résumé
A one-step immunomagnetic separation technique was performed on a microfluidic platform for the isolation of specific cells from blood samples. The cell isolation and purification studies targeted T cells, as a model for low abundance cells (about 1[ratio]10,000 cells), with more dilute cells as the ultimate goal. T cells were successfully separated on-chip from human blood and from reconstituted blood samples. Quantitative polymerase chain reaction analysis of the captured cells was used to characterize the efficiency of T cell capture in a variety of flow path designs. Employing many (4-8), 50 [micro sign]m deep narrow channels, with the same overall cross section as a single, 3 mm wide channel, was much more effective in structuring dense enough magnetic bead beds to trap cells in a flowing stream. The use of 8-multiple bifurcated flow paths increased capture efficiencies from [similar]20 up to 37%, when compared to a straight 8-way split design, indicating the value of ensuring uniform flow distribution into each channel in a flow manifold for effective cell capture. Sample flow rates of up to 3 [micro sign]L min-1 were evaluated in these capture beds.
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Dans
  • Lab on a Chip 4, nº 6 (2004) : 614–618.
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Numéro NPARC12333684
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Identificateur de l’enregistrement2fac873f-70ce-4365-8693-6ffc6aa4e6fa
Enregistrement créé2009-09-10
Enregistrement modifié2020-04-17

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