| Télécharger | - Voir la version finale : Impact of the temperature on the interactions between common variants of the SARS-CoV-2 receptor binding domain and the human ACE2 (PDF, 1 Mo)
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| DOI | Trouver le DOI : https://doi.org/10.1038/s41598-022-15215-5 |
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| Auteur | Rechercher : Forest-Nault, Catherine1; Rechercher : Koyuturk, Izel1; Rechercher : Gaudreault, Jimmy; Rechercher : Pelletier, Alex1; Rechercher : L'Abbé, Denis1; Rechercher : Cass, Brian1; Rechercher : Bisson, Louis1; Rechercher : Burlacu, Alina1; Rechercher : Delafosse, Laurence1; Rechercher : Stuible, Matthew1; Rechercher : Henry, Olivier; Rechercher : De Crescenzo, Gregory; Rechercher : Durocher, Yves1 |
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| Affiliation du nom | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
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| Bailleur de fonds | Rechercher : Natural Sciences and Engineering Research Council of Canada; Rechercher : National Research Council of Canada; Rechercher : Canada First Research Excellence Fund |
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| Format | Texte, Article |
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| Description physique | 11 p. |
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| Résumé | Several key mutations in the Spike protein receptor binding domain (RBD) have been identified to influence its affinity for the human Angiotensin-Converting Enzyme 2 (ACE2). Here, we perform a comparative study of the ACE2 binding to the wild type (Wuhan) RBD and some of its variants: Alpha B.1.1.7, Beta B.1.351, Delta B.1.617.2, Kappa B.1.617.1, B.1.1.7 + L452R and Omicron B.1.1.529. Using a coiled-coil mediated tethering approach of ACE2 in a novel surface plasmon resonance (SPR)-based assay, we measured interactions at different temperatures. Binding experiments at 10 °C enhanced the kinetic dissimilarities between the RBD variants and allowed a proper fit to a Langmuir 1:1 model with high accuracy and reproducibility, thus unraveling subtle differences within RBD mutants and ACE2 glycovariants. Our study emphasizes the importance of SPR-based assay parameters in the acquisition of biologically relevant data and offers a powerful tool to deepen our understanding of the role of the various RBD mutations in ACE2 interaction binding parameters. |
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| Date de publication | 2022-07-07 |
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| Maison d’édition | Nature Research |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | 15215 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 2b12c2d2-c415-4eda-88ff-98fe8308e5ad |
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| Enregistrement créé | 2022-07-11 |
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| Enregistrement modifié | 2023-03-16 |
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