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Localization and speciation of selenium and mercury in Brassica juncea: implications for Se–Hg antagonism

DOITrouver le DOI : https://doi.org/10.1039/b514954a
AuteurRechercher : ; Rechercher : ; Rechercher : 1Identifiant ORCID : https://orcid.org/0000-0002-3349-5535; Rechercher : ; Rechercher : ; Rechercher :
Affiliation
  1. Conseil national de recherches Canada
FormatTexte, Article
Résumé
The occurrence and form of selenium and mercury were investigated in Indian Mustard, Brassica juncea, a selenium accumulating plant, which had been co-exposed to varying concentration levels of these two elements. Plants were grown and exposed in hydroponic solutions. Following exposure, root exudates were collected in fresh solutions and the head-space around the aerial portions of the plants was sampled. These samples and the harvested plant tissues were then processed for determination of Se and Hg-containing compounds. For the plant tissues, roots, stems and leaves were separated and extracted using a sequential procedure that removed water-soluble species, water-soluble proteins, and dodecyl sulfate-soluble proteins. Size exclusion chromatography allowed further fractionation. High molecular-weight selenium/mercury-containing compounds were found primarily in the plant root extract. Evidence suggests that a Se and Hg complex of high molecular weight may be protein associated. For the analysis of exudate solutions, ion-pairing reversed phase chromatography coupled to ICP-MS was used. Multiple selenium and mercury species were detected, with one mercury-containing compound observed eluting near selenocystine. Plant head-space was sampled with solid phase microextraction and analyzed with GC-ICP-MS and GC-TOFMS. Apart from the primary selenium volatiles and elemental mercury, no volatile species simultaneously containing Se and Hg could be detected.
Date de publication
Maison d’éditionRoyal Society of Chemistry
Dans
  • Journal of Analytical Atomic Spectrometry 21, nº 4 : 404–.
Langueanglais
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Numéro NPARC23001832
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Identificateur de l’enregistrement2aa602b4-407c-4bc9-a6ee-0659cb6cd41c
Enregistrement créé2017-04-13
Enregistrement modifié2020-04-22

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