Development of sensitive LCMS methods for the evaluation of excitotoxic amino acids in marine algae

Auteur	Rechercher : Blay, P.¹; Rechercher : Robertson, A.²; Rechercher : Reeves, K.²; Rechercher : Thomas, K. M.²; Rechercher : Chen, Y. M.¹; Rechercher : Quilliam, M.²
Affiliation	Conseil national de recherches Canada. Développement des cultures et des ressources aquatiques Conseil national de recherches Canada. Science des mesures et étalons
Format	Texte, Allocution
Conférence	12th International Conference on Harmful Algae,4-8 September 2006, Copenhagen, Denmark
Sujet	mass spectrometry; liquid chromatography; amino acids; marine algae; algae; excitotoxic
Résumé	A novel hydrophilic interaction liquid chromatography mass spectrometry (HILIC-MS) method was developed for the separation of excitatory amino acids (EAA) including kainic acid, domoic acid, glutamic acid, aspartic acid, ß-oxalylamino-Lalanine and L-ß-methylaminoalanine in a variety of marine algae. Several HILIC stationary phases were assessed during this study including TSK-gel amide-80 (Tosoh), Atlantis HILIC silica (Waters) and the highly polar, zwitterionic ZIC HILIC (SeQuant). In each case, a variety of parameters were optimised including buffer concentration, organic modifiers, ion-pairing agents, pH, column temperature and flow rate. Single ion monitoring (SIM) of the positive and negative parent ions was performed using an API III, API 165 and API 4000 mass spectrometer. Positive and negative MS/MS spectra were obtained for each EAA examined with the API4000 triple quadrupole MS which facilitated optimization of collision energy and declustering potentials of major fragment ions for single and multiple reaction monitoring. All aspects of quantification will be discussed including linearity assessment, level of detection, level of quantification and effects of sample matrix for each EAA under investigation. These studies were applied to purity assessment of algal derived products such as kainic and domoic acid, and excitotoxin identification in seaweeds, diatoms and cyanobacteria
Langue	anglais
Publications évaluées par des pairs	Non
Numéro du CNRC	NRCC 42621 NRC-IMB-1466
Numéro NPARC	3538354
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	270955af-1c38-4094-b1e1-196292ebfe28
Enregistrement créé	2009-03-01
Enregistrement modifié	2020-03-03

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Conseil national de recherches Canada

Date de modification :: 2026-07-21