| DOI | Trouver le DOI : https://doi.org/10.1038/sj.emboj.7600048 |
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| Auteur | Rechercher : Kozlov, Guennadi; Rechercher : De Crescenzo, Gregory1; Rechercher : Lim, Nadia S; Rechercher : Siddiqui, Nadeem; Rechercher : Fantus, Daniel; Rechercher : Kahvejian, Avak; Rechercher : Trempe, Jean-Francois; Rechercher : Elias, Demetra; Rechercher : Ekiel, Irena1; Rechercher : Sonenberg, Nahum; Rechercher : O'Connor-McCourt, Maureen1; Rechercher : Gehring, Kalle |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | genome; mutagenesis; peptides; pharmaceutical; proteins; surface plasmon resonance; tail |
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| Résumé | The C-terminal domain of poly(A)-binding protein (PABC) is a peptide-binding domain found in poly(A)-binding proteins (PABPs) and a HECT (homologous to E6 -AP C-terminus) family E3 ubiquitin ligase. In protein synthesis, the PABC domain of PABP functions to recruit several translation factors possessing the PABP-interacting motif 2 (PAM2) to the mRNA poly(A) tail. We have determined the solution structure of the human PABC domain in complex with two peptides from PABP-interacting protein-1 (Paip1) and Paip2. The structures show a novel mode of peptide recognition, in which the peptide binds as a pair of beta-turns with extensive hydrophobic, electrostatic and aromatic stacking interactions. Mutagenesis of PABC and peptide residues was used to identify key protein -peptide interactions and quantified by isothermal calorimetry, surface plasmon resonance and GST pull-down assays. The results provide insight into the specificity of PABC in mediating PABP-protein interactions |
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| Date de publication | 2004 |
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| Dans | |
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| Note | English14685257 |
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| Langue | anglais |
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| Numéro du CNRC | 46169 |
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| Numéro NPARC | 3539976 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 239599b9-dbcc-44b0-bb52-3aca0e979d4f |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-04-17 |
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