| DOI | Trouver le DOI : https://doi.org/10.1016/j.biomaterials.2012.10.047 |
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| Auteur | Rechercher : Fortier, Charles1; Rechercher : De Crescenzo, Gregory; Rechercher : Durocher, Yves1 |
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| Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
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| Format | Texte, Article |
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| Sujet | transfection; polyethylenimine; non-viral vector; targeting; oriented tethering; epidermal growth factor (EGF) |
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| Résumé | Surface modification of non-viral gene delivery nanocarriers may provide advanced features such as receptor targeting, endosomal escape and nuclear import. We here report the design of a versatile and tunable immobilization protocol to functionalize nanocarriers for improved transient gene expression. Our strategy is based on specific interactions occurring between a coil-tagged ligand and a complementary coil-functionalized nanocarrier. As a proof of concept, targeting of DNA/polyethylenimine polyplexes to the epidermal growth factor receptor of A431 cells was investigated. Coiled-coil-mediated oriented tethering of epidermal growth factor triggered a drastic increase of the internalization rate of the targeted polyplexes. To explore the tunability of our platform, surface density of targeting ligand was varied; our results indicated that the internalization rate varied with the ligand-to-polyplex ratio in a “switch mode” fashion. This work prefigures possible avenues for our coiled-coil platform in multiplex functionalization to address transient gene expression bottlenecks in recombinant protein production. |
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| Date de publication | 2012-11-06 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 53182 |
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| Numéro NPARC | 21268241 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 1c9e37f0-5368-46c5-8d02-fe1ea701247c |
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| Enregistrement créé | 2013-06-07 |
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| Enregistrement modifié | 2020-04-21 |
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