A combined digital microfluidic test for assessing infection and immunity status for viral disease in saliva

Par Conseil national de recherches du Canada

Téléchargement	Voir la version finale : A combined digital microfluidic test for assessing infection and immunity status for viral disease in saliva (PDF, 3.1 Mio) Voir les données supplémentaires : A combined digital microfluidic test for assessing infection and immunity status for viral disease in saliva (PDF, 1.3 Mio)
DOI	Trouver le DOI : https://doi.org/10.1039/D5LC00308C
Auteur	Rechercher : Sathishkumar, N.Identifiant ORCID : https://orcid.org/0000-0003-1174-2256; Rechercher : Camacho Valenzuela, Jose Gilberto Identifiant ORCID : https://orcid.org/0000-0001-7762-1268; Rechercher : Le, Nhung H.; Rechercher : Yong, Anthony K. C.; Rechercher : Rossotti, Martin A.¹; Rechercher : Dahmer, Joshua; Rechercher : Sklavounos, Alexandros A.Identifiant ORCID : https://orcid.org/0000-0003-1495-7156; Rechercher : Plante, Martin¹; Rechercher : Brassard, Daniel²Identifiant ORCID : https://orcid.org/0000-0001-5183-6455; Rechercher : Malic, Lidija²Identifiant ORCID : https://orcid.org/0000-0001-8594-9191; Rechercher : Moraitis, Anna N.²Identifiant ORCID : https://orcid.org/0000-0002-3416-480X; Rechercher : Biga, Ruzica; Rechercher : El Idrissi, Imane; Rechercher : Tanha, Jamshid¹Identifiant ORCID : https://orcid.org/0000-0002-5240-4598; Rechercher : Labrecque, Jean²; Rechercher : Veres, Teodor²Identifiant ORCID : https://orcid.org/0000-0002-9594-8042; Rechercher : Wheeler, Aaron R.Identifiant ORCID : https://orcid.org/0000-0001-5230-7475
Affiliation	Conseil national de recherches Canada. Thérapeutique en santé humaine Conseil national de recherches Canada. Dispositifs médicaux
Bailleur de fonds	Rechercher : National Research Council of Canada; Rechercher : Natural Sciences and Engineering Research Council of Canada; Rechercher : University of Toronto; Rechercher : Consejo Nacional de Humanidades, Ciencias y Tecnologías
Format	Texte, Article
Résumé	Population assessments of infection and immunity status are critical for public health response to infectious disease. Most microfluidic tools are developed to assess one or the other – few assess both. This study introduces a multiplexed, fully automated digital microfluidic (DMF) platform designed to detect viral protein as a proxy for infection status and host IgG and IgA antibodies as a marker for immunity status. SARS-CoV-2 and patient saliva were used as a model system to evaluate the concept. Specifically, the infection assay relied on nanobody-based capture and detection agents specific to SARS-CoV-2 trimeric spike protein, with a limit of detection (LOD) of 3.8 ng mL⁻¹ in saliva. And the immunity relied on monoclonal antibodies for host IgG and IgA specific to SARS-CoV-2 spike S1 domain, with LODs of 4.8 ng mL⁻¹ and 13.3 ng mL⁻¹ in saliva, respectively. Clinical validation in saliva samples from human subjects experiencing symptoms (n = 14) showed strong correlation with PCR and commercial ELISA, achieving 100% sensitivity and 100% specificity for infection detection and 100% sensitivity with 91.7% and 90.9% specificity for host IgG and IgA, respectively. These results highlight potential applications for the new system as a portable diagnostic tool for outbreak surveillance and public health management, as a step toward preparing for the next global pandemic.
Date de publication	2025-05-27
Maison d’édition	The Royal Society of Chemistry
Licence	Creative Commons, Attribution 3.0 non transposé (CC BY 3.0) https://creativecommons.org/licenses/by/3.0/deed.fr
Dans	Lab on a Chip 25, nº 13 (27 mai 2025) : 3197–3207.
Note	Film supplémentaire disponible sur la page web
Langue	anglais
Publications évaluées par des pairs	Oui
Exporter la notice	Exporter en format RIS
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Identificateur de l’enregistrement	17877985-aa1f-40c8-ae73-7b21f19ce434
Enregistrement créé	2025-08-01
Enregistrement modifié	2025-11-03

Date de modification :: 2025-12-12