Isolation and characterization of Methanobacterium espanolae sp. nov., a mesophilic, moderately acidiphilic methanogen

DOI	Trouver le DOI : https://doi.org/10.1099/00207713-40-1-12
Auteur	Rechercher : Patel, G.¹; Rechercher : Sprott, G.¹; Rechercher : Fein, J.
Affiliation	Conseil national de recherches Canada
Format	Texte, Article
Résumé	Bacterial strain GP9T (T = type strain), a nonmotile, nonsporeforming, mesophilic, methanogenic bacterium, was isolated from the primary sludge obtained from the waste treatment facility of a major kraft pulp mill in Canada. Single cells were 6.0 by 0.8 μm and stained gram positive. Growth and methane production occurred only with H2-CO2 as the substrate. Acetate, formate, propionate, butyrate, pyruvate, methanol, or trimethylamine could not serve as a sole source of carbon and energy for growth. The optimum pH for growth was between 5.6 and 6.2; consistent growth and methane production were not observed below pH 4.68. The optimum temperature for growth was 35°C, and little or no growth was observed during incubation at 15 and 50°C. Kanamycin and bacitracin were severe inhibitors of growth and methanogenesis, whereas 100 μM bromoethanesulfonic acid caused 30% inhibition. Supernatant from primary sludge enhanced growth by about 10%. The DNA base composition was 34 mol% guanine plus cytosine. On the basis of physiological characteristics, indirect immunofluorescence typing, and DNA-DNA hybridization studies, the isolate is named Methanobacterium espanolae sp. nov.
Date de publication	1990
Dans	International Journal of Systematic Bacteriology 40, nº 1 (1990) : 12–18.
Langue	anglais
Numéro du CNRC	PATEL1990A NRCC 30887
Numéro NPARC	9388904
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	0fc1e5b5-f206-4d7a-b122-b11a3aab7857
Enregistrement créé	2009-07-10
Enregistrement modifié	2020-03-17

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Date de modification :: 2026-06-10