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Changes in the Atlantic salmon (Salmo salar) epidermal mucus protein compostion following infection by sea lice (Lepeophtheirus salmonis)

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.cbd.2009.02.001
AuteurRechercher : 1; Rechercher : 2
Affiliation
  1. Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
  2. Conseil national de recherches du Canada. Programme d'aide à la recherche industrielle
FormatTexte, Article
Résumé
The mucus protein profile of Atlantic salmon (Salmo salar) and changes due to infection with sea lice (Lepeophtheirus salmonis) were examined. Two-dimensional gel electrophoresis was performed on salmon skin mucus and comparisons between control and infected fish mucus were made. LC MS/MS identified intracellular proteins, calmodulin, actin, and hemopexin and plasma proteins, such as apolipoproteins, lectin, plasminogen and transferrin. Plasma proteins in the mucus may result from either direct expression by epidermal cells, leakage of plasma or via a secondary circulation system. Therefore, RT-PCR was used to measure mRNA of transferrin and lectin in Atlantic salmon skin. Transferrin expression was observed suggesting direct expression by the epidermis. Lectin expression was not detected suggesting another mechanism of entry into mucus, either leakage from plasma or secondary circulation. The lack of observable albumin on 2D gels, suggests that mucus lectin may arise from the secondary circulation route. Interestingly, β-actin was a significant component of Atlantic salmon mucus. Cleaved actin and transferrin fragments were observed and positively correlated with sea lice infection suggestive of proteolytic activity. Increased levels of cleaved transferrin during sea lice infection may activate the nitrous oxide response of salmon macrophages, as part of the fish's immune response to sea lice infection.
Date de publication
Dans
  • Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 4, nº 3 (septembre 2009) : 159–167.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 42799
1784
Numéro NPARC3538240
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Identificateur de l’enregistrement0df5d6b2-34bd-4155-8efc-9fd1c491ee4e
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-16
Date de modification :