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The characterization of the O-antigen of Escherichia coli 064:K99 lipopolysaccharide

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/0008-6215(93)84134-R
AuteurRechercher : ; Rechercher : ; Rechercher :
FormatTexte, Article
Sujetacetylation; antigens; carbohydrate conformation; carbohydrate sequence; chromatography, gel; degradation; growth & development; hydrolysis; immunology; isolation & purification; lipopolysaccharides; lipopolysaccharides, smooth; LPS; magnetic resonance spectroscopy; methylation; molecular sequence data; molecular weight; nuclear magnetic resonance, 1D; nuclear magnetic resonance, 2D; O antigens; O-chain; oligosaccharides; polysaccharides; polysaccharides, bacterial; repeating unit; Escherichia coli
Résumé
The structure of the O-polysaccharide of the smooth lipopolysaccharide (LPS) produced by Escherichia coli O64:K99 was investigated by SDS-PAGE, composition, periodate oxidation, methylation, partial hydrolysis, and 1D and 2D nuclear magnetic resonance analyses, made on the native o-chain and its reduction and periodate degradation products. The E. coli O64 antigenic O-chain was found to be a high molecular weight glycan composed of D-galactose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-mannose (2:1:1:1) and was a polymer of branched pentasaccharide repeating units having the structure: [formula: see text]
Date de publication
Maison d’éditionElsevier
Dans
  • Carbohydrate Research 248, nº 4 October 1993 (4 octobre 1993) : 277–284.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCPERRY1993
Numéro NPARC9375788
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Identificateur de l’enregistrement0c7a6751-947f-4b9c-bcba-fa8eeb3dba0d
Enregistrement créé2009-07-10
Enregistrement modifié2022-11-18
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