| DOI | Trouver le DOI : https://doi.org/10.1016/j.vaccine.2015.05.097 |
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| Auteur | Rechercher : Petiot, Emma; Rechercher : Cuperlovic-Culf, Miroslava1; Rechercher : Shen, Chun Fang2; Rechercher : Kamen, Amine2 |
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| Affiliation | - Conseil national de recherches Canada. Technologies de l'information et des communications
- Conseil national de recherches Canada. Thérapeutique en santé humaine
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| Format | Texte, Article |
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| Résumé | Mammalian cell cultures are increasingly used for the production of complex biopharmaceuticals including viral vectors and vaccines. HEK293 is the predominant cell line used for the transient expression of recombinant proteins and a well-established system for the production of viral vectors. Understanding metabolic requirements for high productivity in HEK293 cells remains an important area of investigation. Many authors have presented approaches for increased productivity through optimization of cellular metabolism from two distinct perspectives. One is a non-targeted approach, which is directed to improving feeding strategies by addition of exhausted or critical substrates and eventually removal of toxic metabolites. Alternatively, a targeted approach has attempted to identify specific targets for optimization through better understanding of the cellular metabolism under different operating conditions. This review will present both approaches and their successes with regards to improvement of viral production in HEK293 cells outlining the key relations between HEK293 cell metabolism and viral vector productivity. Also, we will summarize the current knowledge on HEK293 metabolism indicating remaining issues to address and problems to resolve to maximize the productivity of viral vectors in HEK293 cells. |
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| Date de publication | 2015-11-04 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21275745 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 07914c04-0650-4d1c-b1eb-f0e13aab9cde |
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| Enregistrement créé | 2015-07-14 |
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| Enregistrement modifié | 2020-04-22 |
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