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Composition of Methanospirillum hungatii GP1 during growth on different media

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1139/m80-102
AuteurRechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches Canada
FormatTexte, Article
Résumé
Growth of Methanospirillum hungatii GP1 as determined by optical density measurement was comparable to growth assessed by cell dry weight, ribonucleic acid content, and deoxyribonucleic acid content. Cultivation of M. hungatii on synthetic medium containing mineral salts, vitamins, and acetic acid indicated that, on a dry weight basis, cell constituents such as protein (71%), ribonucleic acid (15.8%), deoxyribonucleic acid (1.6%), and total carbohydrate (3.2%) did not vary significantly with the growth phase. Cells grown in the synthetic medium supplemented with yeast extract and tryptone had slightly higher protein content (76%), but the concentrations of the other cell constituents were similar and did not fluctuate much during growth. Nitrogen limiting growth resulted in somewhat lower ribonucleic acid content as well as slightly higher protein content than that in cells grown in nonlimiting medium. Methanospirillum hungatii did not accumulate any of the commonly known reserve materials under nitrogen or carbon and hydrogen limiting growth.
Date de publication
Dans
  • Canadian Journal of Microbiology 26 (1980) : 577–582.
Langueanglais
Numéro du CNRCBREUIL1980A
NRCC 18121
Numéro NPARC9375706
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Identificateur de l’enregistrement0601c0ac-f1a7-48c7-914b-cee2b546f676
Enregistrement créé2009-07-10
Enregistrement modifié2020-03-13
Date de modification :