| DOI | Resolve DOI: https://doi.org/10.2166/wst.2007.292 |
|---|
| Author | Search for: Guiot, S. R.1; Search for: Kuhn, R.1; Search for: Lévesque, M.-J.1; Search for: Cimpoia, R.1 |
|---|
| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
|
|---|
| Format | Text, Article |
|---|
| Subject | environmental; ultrastructure |
|---|
| Abstract | The electrolytical methanogenic/methanotrophic coupling (eMaMoC) process was tested in a laboratory-scale single-stage reactor for the treatment of tetrachloroethene (PCE)-contaminated waters. A water electrolysis cell was placed directly in the effluent recirculation loop for the supply of both O2 and H2 to the system: H2 serving as the electron donor for both carbonate reduction into CH4 and reductive dechlorination. The concurrent presence of O2 and CH4 could be used by the methanotrophs for co-metabolically oxidising the chlorinated intermediates left over by the anaerobic transformation of PCE. At a PCE inlet of 33-52 mM and a hydraulic residence time (HRT) of 5.6 days, PCE reductive dechlorination to dichloroethene (DCE) was over 95% with a maximum DCE mineralisation of 83%. Fluorescence in situ hybridisation with 16S rRNA probes related to type I and type II methanotrophic bacteria were utilised to localise the methanotrophic communities in the anaerobic/aerobic granules. It evidenced that with operational time, along with increasing oxygenation rate, methanotrophic communities were specifically colonising onto the outermost layer of the anaerobic/aerobic granule. |
|---|
| Publication date | 2007 |
|---|
| In | |
|---|
| Language | English |
|---|
| NRC number | 49031 |
|---|
| NPARC number | 3539932 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | fe76a7d3-b94d-4cc9-9cc1-62acda732375 |
|---|
| Record created | 2009-03-01 |
|---|
| Record modified | 2020-05-10 |
|---|