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Adsorption and uptake of nickel in Methanothrix concilii

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00408304
AuthorSearch for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada
FormatText, Article
Abstract
During growth of the aceticlastic methanogen Methanothrix concilii, 63Ni was incorporated primarily into two soluble proteins, methylcoenzyme M reductase and carbon monoxide dehydrogenase. During short-term studies, an uptake system for nickel was present which saturated, with kinetic constants of apparent K m=91 M and V max=23 nmol/minmg-1 dry wt. Nickel specificity was demonstrated in competition studies, in which magnesium, calcium, or manganese had little influence on 63Ni uptake; cobalt, however, did compete. Although the uptake of nickel was blocked by extreme temperatures (6C or 100C) and by air, the complete abolition of methanogenesis by various ionophores, N,N-dicyclohexylcarbodiimide, bromoethanesulfonate, or pre-treatment of the cells at 80C, had little effect on uptake. The kinetic characteristics of short-term nickel uptake in cells could be reproduced in purified sheath preparations, indicating that the predominant mechanism for nickel uptake in short-term studies was an energy-independent, semi-specific cell-surface absorption.
Publication date
In
  • Archives of Microbiology 150 (1988): 338–342.
LanguageEnglish
NRC numberBAUDET1988
NRCC 29033
NPARC number9363995
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Record identifierfe03a164-2df5-4026-98fc-c7c469e8f282
Record created2009-07-10
Record modified2020-03-17
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