Strategies for augmenting the pentachlorophenol degradation potential of UASB anaerobic granules

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.2166/wst.2002.0283
Author	Search for: Guiot, S. R.¹; Search for: Tartakovsky, B.¹; Search for: Lanthier, M.; Search for: Lévesque, M.-J.¹; Search for: Manuel, M. F.¹; Search for: Beaudet, R.; Search for: Greer, C. W.¹; Search for: Villemur, R.
Affiliation	National Research Council Canada. NRC Biotechnology Research Institute
Format	Text, Article
Conference	9th World Congress on Anaerobic Digestion, September 2-6, 2001, Antwerp, Belgium
Subject	Bacteria, anaerobic; anaerobic granule; bioaugmentation; desulfitobacterium frappieri; FISH; on-line control; PCR; pentachlorophenol; selective stress; biodegradation, environmental; biofilms; biomass; bioreactors; DNA, bacterial; environmental pollutants; gram-positive bacteria; in situ hybridization, fluorescence; particle size; pentachlorophenol; polymerase chain reaction; crossgroup
Abstract	Anaerobic degradation of pentachlorophenol (PCP) is an example of a process that may benefit from enrichment or bioaugmentation. In one approach, enrichment acceleration was attempted by applying an on-line control-based selective stress strategy to a native anaerobic upflow sludge bed (UASB) system; this strategy linked PCP loading rate to methane production. As a result, the reactor biomass potential for PCP complete dechlorination reached a rate of 4 mg g⁻¹ volatile suspended solid (VSS) day⁻¹ within a period of 120 days. In another approach, a pure culture, Desulfitobacterium frappieri PCP-1, a strictly anaerobic Gram-positive bacterium, was used to augment the granular biomass of the UASB reactor. This also resulted in a specific degradation rate of 4 mg PCPg⁻¹ VSS day⁻¹; however, this potential was attained within 56 days. Fluorescent in situ hybridization (FISH) showed that the PCP-1 strain was able to rapidly attach to the granule and densely colonize the outer biofilm layer.
Publication date	2002
Publisher	IWA Publishing
In	Selected proceedings of the 9th World Congress on Anaerobic Digestion: held in Antwerp, Belgium, 2 - 6 September, 2001 (2002): 35–41.
Series	Water Science and Technology 45, no. Anaerobic Digestion IX - Selected 10.
Language	English
Peer reviewed	Yes
NRC number	NRC-BRI-44642 CPENV101
NPARC number	12327871
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Record identifier	fd44dff6-9dc4-45c7-b04d-e712dbc8cea1
Record created	2009-09-10
Record modified	2022-10-28

Date modified:: 2025-12-09