| DOI | Resolve DOI: https://doi.org/10.1002/elps.201400211 |
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| Author | Search for: Guo, Tianyi; Search for: Wei, Yin; Search for: Xu, Changqing; Search for: Watts, Benjamin R.; Search for: Zhang, Zhiyi1; Search for: Fang, Qiyin; Search for: Zhang, Haiying; Search for: Selvaganapathy, P.Ravi; Search for: Deen, M. Jamal |
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| Affiliation | - National Research Council Canada. Information and Communication Technologies
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| Format | Text, Article |
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| Subject | polystyrene; analytical equipment; bacterial count; bacterial strain; bacterium culture; bacterium detection; biochip; concentration (parameters); flow cytometer; flow kinetics; hemocytometer; laboratory device; light scattering; microfluidics; particle size; photonic microfluidic integrated device; signal detection; suspension; Escherichia coli |
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| Abstract | Counting of Escherichia coli DH5α-cell suspensions in PBS is performed using a microflow cytometer based on a photonic-microfluidic integrated device. Side-scattered light signals are used to count the E. coli cells. A detection efficiency of 92% is achieved when compared with the expected count from a hemocytometer. The detection efficiency is correlated to the ratio of sample to sheath flow rates. It is demonstrated that E. coli can be easily distinguished from beads of similar sizes (2-4 μm) as their scattering intensities are different. |
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| Publication date | 2015-01-19 |
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| Publisher | Wiley |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21275809 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | fcc2ed3e-a1ef-4236-a4e9-c81da5bcfdc6 |
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| Record created | 2015-07-14 |
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| Record modified | 2022-11-18 |
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