| DOI | Resolve DOI: https://doi.org/10.1002/biot.200700236 |
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| Author | Search for: Blais, David R.1; Search for: Alvarez-Puebla, Ramon A.2; Search for: Bravo-Vasquez, Juan P.2; Search for: Fenniri, Hicham2; Search for: Pezacki, John Paul1 |
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| Affiliation | - National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
- National Research Council of Canada. National Institute for Nanotechnology
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| Format | Text, Article |
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| Subject | Antigens; barcoded resins; biothreat pathogens; multiplex immunoassays; raman spectroscopy |
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| Abstract | Suspension microsphere immunoassays are rapidly gaining recognition in antigen identification and infectious disease biodetection due to their simplicity, versatility and high-throughput multiplex screening. We demonstrate a multiplex assay based on antibody-functionalized barcoded resins (BCRs) to identify pathogen antigens in complex biological fluids. The binding event of a particular antibody on given bead (fluorescence) and the identification of the specific pathogen agent (vibrational fingerprint of the bead) can be achieved in a dispersive Raman system by exciting the sample with two different laser lines. Anthrax protective antigen, Franciscella tularensis lipopolysaccharide and CD14 antigens were accurately identified and quantified in tetraplex assays with a detection limit of 1 ng/mL. The rapid, versatile and simple analysis enabled by the BCRs demonstrates their potential for multiplex antigen detection and identification in a reconfigurable microarray format. |
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| Publication date | 2008-06-19 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | 531
565 |
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| NPARC number | 12329039 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | fc61b995-6c10-4c60-894e-65202f603161 |
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| Record created | 2009-09-10 |
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| Record modified | 2020-04-15 |
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