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Identification of short protein-destabilizing sequences in Arabidopsis cyclin-dependent kinase inhibitors, ICKs

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1093/jxb/erad411
AuthorSearch for: ; Search for: ORCID identifier: https://orcid.org/0000-0002-5467-7784; Search for: ; Search for: ; Search for: ORCID identifier: https://orcid.org/0000-0001-8861-4645
EditorSearch for: Murray, James
FunderSearch for: Natural Sciences and Engineering Research Council of Canada
FormatText, Article
SubjectArabidopsis; CDK inhibitor; ICK; intrinsically disordered region; KRP; protein degradation; short sequence; ubiquitin-independent
Abstract
Plants have a family of cyclin-dependent kinase (CDK) inhibitors called interactors/inhibitors of CDK (ICKs) or Kip-related proteins (KRPs). ICK proteins have important functions in cell proliferation, endoreduplication, plant growth, and reproductive development, and their functions depend on the protein levels. However, understanding of how ICK protein levels are regulated is very limited. We fused Arabidopsis ICK sequences to green fluorescent protein (GFP) and determined their effects on the fusion proteins in plants, yeast, and Escherichia coli. The N-terminal regions of ICKs drastically reduced GFP fusion protein levels in Arabidopsis plants. A number of short sequences of 10–20 residues were found to decrease GFP fusion protein levels when fused at the N-terminus or C-terminus. Three of the four short sequences from ICK3 showed a similar function in yeast. Intriguingly, three short sequences from ICK1 and ICK3 caused the degradation of the fusion proteins in E. coli. In addition, computational analyses showed that ICK proteins were mostly disordered and unstructured except for the conserved C-terminal region, suggesting that ICKs are intrinsically disordered proteins. This study has identified a number of short protein-destabilizing sequences, and evidence suggests that some of them may cause protein degradation through structural disorder and instability.
Publication date
PublisherOxford Academic
In
  • Journal of Experimental Botany 75, no. 3 (2 February 2024): 772–788.
LanguageEnglish
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

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Record identifierf97048b7-eaa7-4e81-a4f1-017d7c3aba5d
Record created2024-07-23
Record modified2024-08-16
Date modified: