| Alternative title | Characterization of high affinity anti-Internalin B VHH antibody fragments isolated from naturally and artificially immunized repertoires |
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| Download | - View accepted manuscript: High affinity anti-Internalin B VHH antibody fragments isolated from naturally and artificially immunized repertoires (PDF, 2.5 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1016/j.jim.2014.10.009 |
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| Author | Search for: Gene, Robert W.; Search for: Kumaran, Jyothi; Search for: Aroche, Cristina; Search for: van Faassen, Henk1; Search for: Hall, J. Christopher; Search for: Mackenzie, C. Roger1; Search for: Arbabi-Ghahroudi, Mehdi1 |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Subject | Listeria; Camelid; single-domain antibody; phage display; ELISA; surface plasmon resonance |
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| Abstract | The need for rapid and easy technologies for the detection of food-borne and environmental pathogens is essential for safeguarding the health of populations. Furthermore, distribution of tainted food and water can have consequences which can affect whole economies. Antibodies and antibody fragments have been historically used in detection platforms due to their antigen specificity and robust physicochemical properties. In this study, we report the isolation and characterization of antibody fragments from the heavy chain antibody repertoire (VHH) of Camelidae which bind with specificity and high affinity to the Listeria monocytogenes invasin, Internalin B (InlB). To the best of our knowledge, this is the first report of anti-InlB VHHs from camelids. These anti-InlB VHHs were not cross-reactive to the structurally related Listeria invasin Internalin A (InlA) and are potential reagents to be used in the development of detection and medical technologies. |
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| Publication date | 2014-11-08 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-HHT-53221 |
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| NPARC number | 21275314 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | f911f714-77af-4d0a-8db1-9a52916da470 |
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| Record created | 2015-06-05 |
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| Record modified | 2020-06-04 |
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