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Isolation, characterization and expression analysis of a starch branching enzyme II cDNA from wheat

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/S0168-9452(96)04543-8
AuthorSearch for: 1; Search for: 1; Search for: ; Search for: 1; Search for: 1
Affiliation
  1. National Research Council Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectwheat (Triticum aestivum L.); starch branching enzyme; cDNA; gene expression
Abstract
A full-length cDNA (2970 bp) encoding a starch branching enzyme II (SBEII; EC 2.4.1.18) in wheat (Triticum aestivum L. cv Fielder) kernel was isolated from a cDNA library. The translated region of the cDNA predicted a 823 amino acid primary product with a molecular mass of 91.4 kDa. A 54 amino acid transit peptide was postulated to be cleaved from the pre-protein to give a 769 amino acid (85.4 kDa) mature polypeptide, which showed extensive sequence similarity to SBEII sequences characterized from maize, rice and pea. Expression of the isolated cDNA in a BE-deficient E. coli strain demonstrated that it encoded a functional BE. RNA analysis of Sbe2 gene expression during seed development revealed that Sbe2 mRNA levels were highest in young kernels (5–10 days post-anthesis) and declined as the kernels matured.
Publication date
PublisherElsevier
In
  • Plant Science 122, no. 2 (February 1997): 153–163.
LanguageEnglish
Peer reviewedYes
IdentifierS0168945296045438
NRCC-40023
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Record identifierf8068bbe-fcff-4c5a-8d96-a5bdf046cd0f
Record created2023-11-01
Record modified2023-11-01
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