A scalable process for helper-dependent adenoviral vector production using pei-derived transfection strategy in suspension culture

From National Research Council Canada

DOIResolve DOI: http://doi.org/10.1007/978-90-481-3419-9_77
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Name affiliation
  1. National Research Council Canada. NRC Biotechnology Research Institute
TypeArticle
Proceedings titleCells and Culture: Proceedings of the 20th ESACT Meeting, Dresden, Germany, June 17-20, 2007
Series titleESACT Proceedings; no. Vol. 4
Conference20th ESACT Meeting, June 17-20, 2007, Dresden, Germany
ISBN9789048134199
9048134196
Pages441–445
Subjecthelper-dependent adenoviral vector; HEK 293; PEI-transfection; suspension cells; bioreactor
Abstract
Helper-dependent adenoviral vector has demonstrated a high therapeutic potential; however, the poor efficiency of the actual production process hampers further large-scale clinical evaluation. In this work, we evaluated the adenofection protocol, an efficient polyethylenimine transfection strategy, for HDV production. Optimized conditions of PEI-adenofection were used to generate HDV at 3-L bioreactor scale. This process strategy realized in suspension reduced process duration and therefore ensured production of high-quality vector stock.
Publication date
PublisherSpringer
LanguageEnglish
Peer reviewedYes
NPARC number23001980
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