| DOI | Resolve DOI: https://doi.org/10.1007/978-90-481-3419-9_77 |
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| Author | Search for: Dormond, E.1; Search for: Meneses-Acosta, A.1; Search for: Jacob, D.1; Search for: Durocher, Y.1; Search for: Perrier, M.; Search for: Kamen, A.1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Conference | 20th ESACT Meeting, June 17-20, 2007, Dresden, Germany |
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| Subject | helper-dependent adenoviral vector; HEK 293; PEI-transfection; suspension cells; bioreactor |
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| Abstract | Helper-dependent adenoviral vector has demonstrated a high therapeutic potential; however, the poor efficiency of the actual production process hampers further large-scale clinical evaluation. In this work, we evaluated the adenofection protocol, an efficient polyethylenimine transfection strategy, for HDV production. Optimized conditions of PEI-adenofection were used to generate HDV at 3-L bioreactor scale. This process strategy realized in suspension reduced process duration and therefore ensured production of high-quality vector stock. |
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| Publication date | 2010-06-02 |
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| Publisher | Springer |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23001980 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | f7673588-623f-4a5b-8cd5-0cedb223d1d6 |
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| Record created | 2017-07-12 |
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| Record modified | 2020-04-17 |
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