Multilocus sequence typing and virulence gene profiles associated with Escherichia coli from human and animal sources

From National Research Council Canada

DOIResolve DOI: http://doi.org/10.1089/fpd.2014.1860
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Name affiliation
  1. National Research Council Canada. Human Health Therapeutics
TypeArticle
Journal titleFoodborne Pathogens and Disease
ISSN1535-3141
Volume12
Issue4
Pages302–310; # of pages: 9
Subjectanimal food; bacterial virulence; bacterium isolate; beef cattle meat; beef meat; chicken meat; controlled study; Escherichia coli; hospital infection; human; meat; multidimensional scaling; multilocus sequence typing; nonhuman; pathotype; pig meat; pork meat; Escherichia coli
Abstract
We investigated whether specific sequence types, and their shared virulence gene profiles, may be associated with both human and food animal reservoirs. A total of 600 Escherichia coli isolates were assembled from human (n=265) and food-animal (n=335) sources from overlapping geographic areas and time periods (2005-2010) in Canada. The entire collection was subjected to multilocus sequence typing and a subset of 286 E. coli isolates was subjected to an E. coli-specific virulence gene microarray. The most common sequence type (ST) was E. coli ST10, which was present in all human and food-animal sources, followed by ST69, ST73, ST95, ST117, and ST131. A core group of virulence genes was associated with all 10 common STs including artJ, ycfZ, csgA, csgE, fimA, fimH, gad, hlyE, ibeB, mviM, mviN, and ompA. STs 73, 92, and 95 exhibited the largest number of virulence genes, and all were exclusively identified from human infections. ST117 was found in both human and food-animal sources and shared virulence genes common in extraintestinal pathogenic E. coli lineages. Select groups of E. coli may be found in both human and food-animal reservoirs.
Publication date
PublisherMary Ann Liebert Inc.
LanguageEnglish
Peer reviewedYes
NPARC number21275695
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Record created2015-07-14
Record modified2016-05-09
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