Multilocus sequence typing and virulence gene profiles associated with Escherichia coli from human and animal sources

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1089/fpd.2014.1860
Author	Search for: Manges, Amee R.; Search for: Harel, Josée; Search for: Masson, Luke¹; Search for: Edens, Thaddeus J.; Search for: Portt, Andrea; Search for: Reid-Smith, Richard J.; Search for: Zhanel, George G.; Search for: Kropinski, Andrew M.; Search for: Boerlin, Patrick
Name affiliation	National Research Council of Canada. Human Health Therapeutics
Format	Text, Article
Journal title	Foodborne Pathogens and Disease
ISSN	1535-3141
Volume	12
Issue	4
Pages	302–310
Subject	animal food; bacterial virulence; bacterium isolate; beef cattle meat; beef meat; chicken meat; Escherichia coli; hospital infection; meat; multidimensional scaling; multilocus sequence typing; pathotype; pig meat; pork meat; Escherichia coli
Abstract	We investigated whether specific sequence types, and their shared virulence gene profiles, may be associated with both human and food animal reservoirs. A total of 600 Escherichia coli isolates were assembled from human (n=265) and food-animal (n=335) sources from overlapping geographic areas and time periods (2005-2010) in Canada. The entire collection was subjected to multilocus sequence typing and a subset of 286 E. coli isolates was subjected to an E. coli-specific virulence gene microarray. The most common sequence type (ST) was E. coli ST10, which was present in all human and food-animal sources, followed by ST69, ST73, ST95, ST117, and ST131. A core group of virulence genes was associated with all 10 common STs including artJ, ycfZ, csgA, csgE, fimA, fimH, gad, hlyE, ibeB, mviM, mviN, and ompA. STs 73, 92, and 95 exhibited the largest number of virulence genes, and all were exclusively identified from human infections. ST117 was found in both human and food-animal sources and shared virulence genes common in extraintestinal pathogenic E. coli lineages. Select groups of E. coli may be found in both human and food-animal reservoirs.
Publication date	2015-04-02
Publisher	Mary Ann Liebert Inc.
Language	English
Peer reviewed	Yes
NPARC number	21275695
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Record identifier	f7555975-1e81-48e4-b7d3-db4c9b467b64
Record created	2015-07-14
Record modified	2020-04-22

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Date modified:: 2020-09-28