| Abstract | A predominantly haploid, approx. 57%, suspension culture of Datura innoxia was obtained and conditions for the isolation, culture and regeneration to shoots of protoplasts from these cells are described.
Chromosome counts of protoplast-derived cultures showed that no haploids were present among 15 regenerated shoots, and only 3 of 30 calli contained any haploid tissue. Approx. 9.5% of mitoses examined in a protoplast-derived suspension culture were haploid.
The presence of haploid sets of diplochromosomes in the early divisions of protoplasts suggests that endomitosis may be one cause for the observed ploidy increase. |
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