Cryopreservation of immature spring wheat zygotic embryos using an abscisic acid pretreatment

DOI	Resolve DOI: https://doi.org/10.1007/BF00241941
Author	Search for: Kendall, Edward J.¹; Search for: Kartha, Kutty K.¹; Search for: Qureshi, Javed A.¹; Search for: Chermak, Paul¹
Affiliation	National Research Council Canada. NRC Plant Biotechnology Institute
Format	Text, Article
Subject	cryopreservation; Triticum aestivum; abscisic acid
Abstract	Spring wheat (Triticum aestivum L.) zygotic embryos were successfully cryopreserved, without the addition of exogenous cryoprotectants, using only an abscisic acid (ABA) pretreatment. Optimum survival was obtained when embryos were cultured in vitro for 10 days on semisolid Murashige and Skoog (MS) nutrient medium supplemented with 0.5 mg/L (±) ABA prior to cryopreservation. The embryos resumed growth within three days when returned to MS medium devoid of ABA but containing 2mg/L 2,4-dichlorophenoxyacetic acid. The embryogenic calli produced from these embryos exhibited normal plant regeneration on auxin-free media. Changes in dw/fw ratio, as well as the esterified fatty acid and sucrose concentrations correlated positively with the development of tolerance to cryopreservation.
Publication date	1993-01
Publisher	Springer Nature
In	Plant Cell Reports 12, no. 2 (January 1993).
Language	English
Peer reviewed	Yes
Identifier	BF00241941 NRCC-33519
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Record identifier	f2b8ba3a-0eb9-450a-b860-b25b9fa1cd03
Record created	2023-11-06
Record modified	2023-11-06