| Alternative title | The early response of Atlantic salmon (Salmo salar L) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish |
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| Author | Search for: Ewart, K. Vanya1; Search for: Williams, Jason1; Search for: Richards, Robert C.1; Search for: Gallant, Jeffrey W.1; Search for: Melville, Krista1; Search for: Douglas, Susan E.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Marine Biosciences
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| Format | Text, Article |
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| Subject | inflammation; acute phase response; fish; microarray; qPCR |
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| Abstract | Aeromonas salmonicida is a fish pathogen that causes furunculosis. Virulent strains of this bacterium are able to infect salmonid macrophages and survive within them, although mechanisms favouring intracellular survival are not completely understood. It is known that A. salmonicida cultured in vivo in the peritoneal cavity of the host undergoes changes in gene expression and surface architecture compared with cultures grown in vitro in broth. Therefore, in this study, the early macrophage responses to A. salmonicida grown in vivo and in vitro were compared. Macrophage-enriched cell preparations from head kidney of Atlantic salmon (Salmo salar) were infected in vitro in 96-well microtitre dishes and changes in gene expression during the infection process were monitored using a custom Atlantic salmon cDNA microarray. A. salmonicida cultures grown in tryptic soy broth and in peritoneal implants were used to infect the macrophages. The macrophages were harvested at 0.5, 1.0 and 2.0 h after addition of the bacteria to the medium. Significant changes in gene expression were evident by microarray analysis at 2.0 h post-infection in macrophages infected with broth-grown and implant-grown bacteria; however, qPCR analysis revealed earlier up-regulation of JunB and TNF-α in macrophages exposed to the implant-grown bacteria. Up-regulation of those genes and others is consistent with the effects of extracellular products of aeromonad bacteria on macrophages and also suggests initiation of the innate immune response. |
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| Publication date | 2007-08-20 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | 1705
NRCC 42746 |
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| NPARC number | 3538120 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | f29fc1f2-3e48-4dfa-8001-12d683a20318 |
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| Record created | 2009-03-01 |
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| Record modified | 2020-05-10 |
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