Trace determination of domoic acid in sea water and phytoplankton by high-performance liquid chromatography of the gluorenylmethoxycarbonyl (FMOC) derivative

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1080/03067319008026940
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Name affiliation
  1. National Research Council of Canada
FormatText, Article
Journal titleInternational Journal of Environmental Analytical Chemistry
ISSN0306-7319
Volume38
Issue3
Pages351–368
Subjectdomoic acid; neurotoxin; FMOC derivatization; reversed-phase HPLC; fluorescence detection; phytoplankton; Nitzschia pungens
Abstract
A method is presented for the trace determination of domoic acid, a neurotoxic amino acid responsible for cases of Amnesic Shellfish Poisoning resulting from the consumption of contaminated shellfish. The method involves pre-column derivatization with 9-fluorenylmethylchloroformate to form the FMOC derivative followed by reversed-phase HPLC with fluorescence detection. The detection limit for domoic acid in seawater and aqueous extracts is 15pg/mL (50 pM) using gradient elution, a 20μL injection volume, and a 2.1mm I.D. microbore column. Use of dihydrokainic acid as an internal standard improved quantitation. The method was applied to the detection of domoic acid in seawater, in phytoplankton cultures (Nitzschia pungens forma multiseries), and in natural mixed phytoplankton assemblages in estuarine waters.
Publication date
PublisherTaylor and Francis
LanguageEnglish
Peer reviewedYes
NPARC number23000944
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