| DOI | Resolve DOI: https://doi.org/10.1080/03067319008026940 |
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| Author | Search for: Pocklington, R.; Search for: Milley, J. E.1; Search for: Bates, S. S.1; Search for: Bird, C. J.1; Search for: De freitas, A. S. W.; Search for: Quilliam, M. A.1 |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Subject | domoic acid; neurotoxin; FMOC derivatization; reversed-phase HPLC; fluorescence detection; phytoplankton; Nitzschia pungens |
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| Abstract | A method is presented for the trace determination of domoic acid, a neurotoxic amino acid responsible for cases of Amnesic Shellfish Poisoning resulting from the consumption of contaminated shellfish. The method involves pre-column derivatization with 9-fluorenylmethylchloroformate to form the FMOC derivative followed by reversed-phase HPLC with fluorescence detection. The detection limit for domoic acid in seawater and aqueous extracts is 15pg/mL (50 pM) using gradient elution, a 20μL injection volume, and a 2.1mm I.D. microbore column. Use of dihydrokainic acid as an internal standard improved quantitation. The method was applied to the detection of domoic acid in seawater, in phytoplankton cultures (Nitzschia pungens forma multiseries), and in natural mixed phytoplankton assemblages in estuarine waters. |
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| Publication date | 1990-03-01 |
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| Publisher | Taylor and Francis |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23000944 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | f22912ef-aaf2-40ee-b581-6eb9362f3264 |
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| Record created | 2016-11-21 |
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| Record modified | 2020-03-17 |
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