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Transient expression and purification of chimeric heavy chain antibodies

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.pep.2008.10.011
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Affiliation
  1. National Research Council Canada. NRC Institute for Biological Sciences
  2. National Research Council Canada. NRC Biotechnology Research Institute
FormatText, Article
Subject(chimeric) heavy chain antibody; antibody expression; single domain antibody; transient expression; biotechnology; in vitro; protein; glycosylation; antibodies, monoclonal; antibody; assay; biosynthesis; camelids, new world; Canada; cell line, tumor; chain; domain; efficient; gene expression; genetics; immunoglobulin heavy chains; monoclonal-antibody; recombinant fusion proteins
Abstract
Monoclonal antibodies have been successfully engineered as approved therapeutics. However, their large size is considered a major factor preventing them from having a more efficient tissue penetration. As the first step to establish a possibly more efficient antibody platform, we present here transient expression, purification and characterization of six chimeric heavy chain antibodies (cHCAbs), or fusion of camelid single domain antibodies (sdAbs) to human fragment crystallizable (Fc). All six HCAbs have a MW of not, vert, similar80 kDa, expressed well in a HEK293 expression system and have G0, G1 and G2 types of glycosylation. The transient expression also provided a very fast way to generate high milligram to low gram amount of proteins for in vitro assays and preliminary animal studies.
Publication date
In
  • Protein Expression and Purification 65, no. 1 (26 October 2008): 77–82.
LanguageEnglish
Peer reviewedYes
NRC numberZHANG2009A
NRCC 47831
NPARC number15329265
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Record identifierf0c1afa1-4615-44af-860d-1c805cbb1943
Record created2010-05-21
Record modified2020-04-15
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