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Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.)

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.cbpb.2006.04.011
AuthorSearch for: 1; Search for: 1; Search for: ; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Institute for Marine Biosciences
FormatText, Article
Subjectastaxanthin-binding; Atlantic halibut; Atlantic salmon; fluorescence intensity; haddock; muscle proteins
Abstract
A study was conducted to compare astaxanthin binding ability of solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Muscle proteins of juvenile Atlantic salmon, haddock and halibut were solubilized by sequential extraction of muscle tissue using low ionic strength solutions. Electrophoretic protein profiles of the six solubilized fractions from these species were similar. Each solubilized fraction from the three species was examined for its relative astaxanthin binding capacity. The amount of bound astaxanthin was significantly different (P < 0.05) among the six fractions of each species. Significant differences in astaxanthin binding were only found for fractions A and E among the species. The amount of bound astaxanthin in various fractions of each species showed a good correlation (R2 = 0.80–0.92) with the ANS (8-anilino-1-naphthalenesulfonate) fluorescence intensity of those fractions. The pattern and extent of astaxanthin binding to the muscle proteins of juvenile salmon, haddock and halibut is comparable to that reported previously for adult Atlantic salmon [Saha, M.R., Ross, N.W., Gill, T.A., Olsen, R.E., Lall, S.P., 2005. Development of a method to assess binding of astaxanthin to Atlantic salmon S. salar L. muscle proteins. Aquacult. Res. 36, 336–343.]. These combined observations suggest that the carotenoid binding capacity of the muscle proteins of salmon is not the limiting factor in the deposition of carotenoid in their flesh.
Publication date
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  • Crown Copyright© 2006 Published by Elsevier Inc. All rights reserved.
In
  • Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 144, no. 4 (4 May 2006): 488–495.
LanguageEnglish
Peer reviewedYes
NRC number1617
NRCC 55556
NPARC number3538239
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Record identifieref198444-b6e0-4483-9d50-6105ea75423b
Record created2009-03-01
Record modified2020-04-22
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