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Cloning of a cDNA encoding the 21.2 kDa oleosin isoform from Arabidopsis thaliana and a study of its expression in a mutant defective in diacylglycerol acyltransferase activity

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00021805
AuthorSearch for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectoleosin; cDNA; Arabidopsis thaliana; diacylglycerol acyltransferase
Abstract
A full-length cDNA clone (pA23) of 832 bp encoding an oleosin from Arabidopsis thaliana was isolated by differential screening of a silique-specific cDNA library with probes prepared from poly(A)⁺ RNA isolated from developing seeds of wild-type (WT) Arabidopsis and from mutant AS11 with a lesion affecting diacylglycerol acyltransferase (DGAT) activity during embryo development. The encoded protein has a calculated molecular mass of 21.2 kDa, and its amino acid sequence shows strong sequence homology and structural similarity to other known oleosins. Transcription of the oleosin gene during seed development was both reduced and delayed in AS11 compared to WT. However, the level of oleosin protein did not appear to be down-regulated during seed development, and at maturity, the overall level of oleosin protein was similar in both WT and AS11. These findings indicate that regulation of oleosin gene expression is part of a highly complex, and co-ordinated expression of storage lipid biosynthesis and related (oleosin) genes during oilseed development.
Publication date
PublisherSpringer Link
In
  • Plant Molecular Biology 31, no. 2 (May 1996): 429–433.
LanguageEnglish
Peer reviewedYes
IdentifierBF00021805
NRCC-38925
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Record identifieree605cd6-1efe-4b84-8bb0-a7afdd4f58aa
Record created2023-11-02
Record modified2023-11-02
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