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Isolation and structure of an acetolactate synthase gene from Schizosaccharomyces pombe and complementation of the ilv2 mutation in Saccharomyces cerevisiae

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00351720
AuthorSearch for: 1; Search for: ; Search for: 1
Affiliation
  1. National Research Council Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectacetolactate synthase; complementation; Schizosaccharomyces pombe; gene cloning
Abstract
A gene encoding a functional acetolactate synthase (ALS) subunit has been isolated from the fission yeast Schizosaccharomyces pombe, and has been structurally and genetically characterized. The approximate 5-kbp cloned DNA segment was found to contain a 2007-bp open reading frame capable of encoding a 669 aminoacid polypeptide which exhibited 57.1% similarity to the corresponding ALS subunit from Saccharomyces cerevisiae. The putative ilv1 isolated from S. pombe was shown to encode a functional subunit of acetolactate synthase by complementation of an S. cerevisiae strain deleted for the ILV2 locus.
Publication date
PublisherSpringer Nature
In
  • Current Genetics 24, no. 6 (December 1993): 544–547.
LanguageEnglish
Peer reviewedYes
IdentifierBF00351720
NRCC-37330
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Record identifierec4bd62e-e081-47a8-9d0a-b823673fa36f
Record created2023-11-06
Record modified2023-11-06
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