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The core and O-polysaccharide structure of the Caulobacter crescentus lipopolysaccharide

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.carres.2014.10.003
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Affiliation
  1. National Research Council of Canada. Human Health Therapeutics
FormatText, Article
SubjectChemical analysis; Gene encoding; Nuclear magnetic resonance; Chemical method; Core oligosaccharide; Extracellular polysaccharides; Lipopolysaccharides; Mild acid hydrolysis; O-polysaccharide structure; Periodate oxidation; 3 methylglucose; 4 amino 4 deoxy d rhamnose; guanosine diphosphate fucose; guanosine diphosphate fucose synthase; lipopolysaccharide; mannose; O antigen; oligosaccharide; periodate; polysaccharide; rhamnose; bacterial polysaccharide; bacterium lipopolysaccharide; perosamine; rhamnan; carbohydrate analysis; Caulobacter crescentus; hydrolysis; membrane damage; oxidation; proton nuclear magnetic resonance; carbohydrate synthesis; Caulobacter vibrioides
Abstract
Here we describe the analysis of the structure of the lipopolysaccharide (LPS) from Caulobacter crescentus strain JS1025, a derivative of C. crescentus CB15 NA1000 with an engineered amber mutation in rsaA, leading to the loss of the protein S-layer and gene CCNA-00471 encoding a putative GDP-l-fucose synthase. LPS was isolated using an aqueous membrane disruption method. Polysaccharide and core oligosaccharide were produced by mild acid hydrolysis and analyzed by nuclear magnetic resonance spectroscopy and chemical methods. Spectra revealed the presence of two polysaccharides, one of them, a rhamnan, could be removed using periodate oxidation. Another polymer, built from 4-amino-4-deoxy-d-rhamnose (perosamine), mannose, and 3-O-methyl-glucose, should be the O-chain of the LPS according to genetic data. The attribution of the rhamnan as a part of LPS or a separate polymer was not possible.
Publication date
In
  • Carbohydrate Research 402 (24 October 2014): 111–117.
LanguageEnglish
Peer reviewedYes
NPARC number21275679
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Record identifierebb93d00-6dce-4910-8908-f36ec82154fd
Record created2015-07-14
Record modified2020-04-22
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