Evaluation of protein separation mechanism and pore size distribution in colloidal self-assembled nanoparticle sieves for on-chip protein sizing

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1002/elps.201600339
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Name affiliation
  1. National Research Council of Canada. Nanotechnology
FormatText, Article
Journal titleELECTROPHORESIS
ISSN0173-0835
1522-2683
Volume38
Issue2
Pages342–349
Subjectcolloidal self assembly; microfluidics; nanofluidics; nanoporous media; protein separation
Abstract
The separation behavior of 6.5–66 kDa proteins in silica particle array‐based sieves formed by colloidal self‐assembly in microchips is reported across a pore size range of 22–103 nm. The protein separation and resolution improves markedly with decreasing pore size. The variation of electrophoretic mobility with molecular weight of SDS–protein complexes and with particle size was evaluated using the Ogston sieving equation for a random pore gel structure, and using the modified Giddings equation developed by Wirth for uniform pore structures. The Wirth/Giddings equation provides the best fit for estimation of molecular weight of proteins, and demonstrates that even though experimental evidence shows there is some dispersion in measured pore sizes, these structures can best be described as having a uniform pore size.
Publication date
PublisherWiley
LanguageEnglish
Peer reviewedYes
NPARC number23003062
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