The effect of canavanine on the growth of cells from suspension cultures and on intergeneric heterokaryocytes of canavanine sensitive and tolerant plants

DOI	Resolve DOI: https://doi.org/10.1016/S0015-3796(17)30128-2
Author	Search for: Constabel, F.¹; Search for: Kirkpatrick, J.W.¹; Search for: Kao, K.N.¹; Search for: Kartha, K.K.¹
Affiliation	National Research Council of Canada. Prairie Regional Laboratory
Format	Text, Article
Subject	cell culture; cell fusion; heterokaryocytes; effect of canavanine; incompatibility; leguminosae
Abstract	Through application of the polyethylene glycol method, protoplasts of canavanine sensitive soybean cell cultures were fused with canavanine tolerant protoplasts from alfalfa, Caragana, and sweet clover. Within several days of culture the fusion products, heterokaryocytes, divided and developed into small cell clusters. The heterokaryocytes exhibited a sensitivity to canavanine as strong as soybean protoplasts. The results indicate that protoplasts from cultured soybean cells and from mature leaves of alfalfa, Caragana,and sweet clover when fused do not generate an incompatibility which would prevent cell division. The reaction pattern of the heterokaryocytes appeared to be determined by the soybean partner.
Publication date	1975
Publisher	Elsevier
In	Biochemie und Physiologie der Pflanzen 168, no. 1-4 (1975): 319–325.
Language	English
Peer reviewed	Yes
Identifier	S0015379617301282
NRC number	NRCC 14920
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Record identifier	e852a3b5-782d-419e-a6e6-16ddc15aae74
Record created	2023-07-27
Record modified	2023-07-27