| DOI | Resolve DOI: https://doi.org/10.1007/978-1-0716-0795-4_30 |
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| Author | Search for: Akache, Bassel1; Search for: McCluskie, Michael J.1 |
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| Affiliation | - National Research Council Canada. Human Health Therapeutics
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| Format | Text, Book Chapter |
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| Subject | vaccines; ELISpot; T lymphocyte; Interferon-γ; ovalbumin |
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| Abstract | The enzyme-linked immune absorbent spot (ELISpot) assay allows for the quantification of the number of cells producing a particular secreted analyte. As T lymphocytes secrete cytokines such as interferon (IFN)-γ upon binding of the T cell receptor with its cognate antigen epitope, IFN-γ ELISpot allows for the measurement of antigen-specific T cells in an immune sample. Immune cells are isolated from the vaccinated subject and incubated with the epitope/antigen of interest on polyvinylidene difluoride (PVDF)-lined microplates precoated with a capture antibody to IFN-γ. Cytokine spots are then detected utilizing an IFN-γ-specific detection antibody and an enzyme-linked conjugate. Here, we describe the quantification of OVA-specific CD8 and CD4 T cells from mouse splenocytes to measure vaccine-induced cellular responses. |
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| Publication date | 2020-09-22 |
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| Publisher | Humana |
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| In | |
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| Series | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-HHT_53443 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | e3474a32-6573-4d48-97a5-cfe0b83efbc3 |
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| Record created | 2021-01-09 |
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| Record modified | 2021-01-10 |
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