Analysis of paralytic shellfish poisoning toxins by automated pre-column oxidation and microcolumn liquid chromatography with fluorescence detection

DOI	Resolve DOI: https://doi.org/10.1016/0021-9673(93)80715-K
Author	Search for: Janeček, Mojmir¹; Search for: Quilliam, Michael A.¹; Search for: Lawrence, James F.
Name affiliation	National Research Council Canada. NRC Institute for Marine Biosciences
Format	Text
Type	Article
Journal title	Journal of Chromatography A
ISSN	00219673
Volume	644
Issue	2
Pages	321–331
Abstract	Periodate oxidation of the toxins responsible for paralytic shellfish poisoning (PSP) yields fluorescent purines suitable for trace analysis by reversed-phase LC. Mobile phases containing perfluorinated acids, such as heptafluorobutyric acid, as ion-pair agents were found to provide high capacity factors for the oxidized products. Gradient elution on a microbore column with large volume injections and fluorescence detection permitted the detection of femtomole quantities of PSP toxins. A fully automated pre-column oxidation procedure was developed for an LC autosampler system in order to improve precision and allow unattended analyses. The complete method was applied successfully to various samples, including shellfish and toxic phytoplankton.
Publication date	1993-08
Publisher	ScienceDirect
Language	English
Peer reviewed	Yes
NPARC number	23000963
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Record identifier	e1c0239a-6005-4338-baad-b65c716fbb10
Record created	2016-11-22
Record modified	2016-11-22