Analysis of paralytic shellfish poisoning toxins by automated pre-column oxidation and microcolumn liquid chromatography with fluorescence detection

From National Research Council Canada

DOIResolve DOI: http://doi.org/10.1016/0021-9673(93)80715-K
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Name affiliation
  1. National Research Council Canada. NRC Institute for Marine Biosciences
TypeArticle
Journal titleJournal of Chromatography A
ISSN00219673
Volume644
Issue2
Pages321–331
Abstract
Periodate oxidation of the toxins responsible for paralytic shellfish poisoning (PSP) yields fluorescent purines suitable for trace analysis by reversed-phase LC. Mobile phases containing perfluorinated acids, such as heptafluorobutyric acid, as ion-pair agents were found to provide high capacity factors for the oxidized products. Gradient elution on a microbore column with large volume injections and fluorescence detection permitted the detection of femtomole quantities of PSP toxins. A fully automated pre-column oxidation procedure was developed for an LC autosampler system in order to improve precision and allow unattended analyses. The complete method was applied successfully to various samples, including shellfish and toxic phytoplankton.
Publication date
PublisherScienceDirect
LanguageEnglish
Peer reviewedYes
NPARC number23000963
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Record identifiere1c0239a-6005-4338-baad-b65c716fbb10
Record created2016-11-22
Record modified2016-11-22
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