| DOI | Resolve DOI: https://doi.org/10.1016/0378-4347(93)80446-B |
|---|
| Author | Search for: Janeček, Mojmir; Search for: Quilliam, Michael A.1; Search for: Bailey, M. Ruth; Search for: North, David H. |
|---|
| Affiliation | - National Research Council Canada. NRC Institute for Marine Biosciences
|
|---|
| Format | Text, Article |
|---|
| Abstract | The chromatographic performance of erythromycin A (EA) is improved significantly over that achieved on polymeric columns by using a sterically shielded octyldiisopropylsilica (Zorbax Rx-C8) column and a neutral mobile phase consisting of 5 mM aqueous sodium perchlorate—acetonitrile (50:50). This mobile phase facilitates electrochemical detection of EA at the 3-pmol level. Temperature control of both column and detection cell is important for minimizing detector noise and drift. A clean-up procedure, based on aminopropylsilica solid-phase extraction, allows the detection of EA in salmon flesh down to the 0.2-ppm level. Some of the metabolites of EA that retain the tertiary amine may also be detected by this method. |
|---|
| Publication date | 1993-09 |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NPARC number | 23001019 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | e16ee5b1-84b3-478a-886e-6d9991d23d12 |
|---|
| Record created | 2016-11-29 |
|---|
| Record modified | 2020-04-24 |
|---|
