Cellular delivery of plasmid DNA into wheat microspores using rosette nanotubes

From National Research Council Canada

Download
  1. (PDF, 2 MB)
  2. (PDF, 668 KB)
DOIResolve DOI: https://doi.org/10.1021/acsomega.0c02830
AuthorSearch for: 1; Search for: 2; Search for: 2; Search for: 2; Search for: 1; Search for: 2; Search for: 1ORCID identifier: https://orcid.org/0000-0001-7629-7080; Search for: 2ORCID identifier: https://orcid.org/0000-0001-5151-9484
Name affiliation
  1. National Research Council of Canada. Nanotechnology
  2. National Research Council of Canada. Aquatic and Crop Resource Development
FormatText, Article
Journal titleACS Omega
ISSN2470-1343
2470-1343
Volume5
Issue38
Pages24422–24433
Subjectgenetics; monomers; organic polymers; fluorescence; gene delivery
Abstract
Plant genetic engineering offers promising solutions to the increasing demand for efficient, sustainable, and high-yielding crop production as well as changing environmental conditions. The main challenge for gene delivery in plants is the presence of a cell wall that limits the transportation of genes within the cells. Microspores are plant cells that are, under the right conditions, capable of generating embryos, leading to the formation of haploid plants. Here, we designed cationic and fluorescent rosette nanotubes (RNTs) that penetrate the cell walls of viable wheat microspores under mild conditions and in the absence of an external force. These nanomaterials can capture plasmid DNA to form RNT–DNA complexes and transport their DNA cargo into live microspores. The nanomaterials and the complexes formed were nontoxic to the microspores.
Publication date
PublisherAmerican Chemical Society
LanguageEnglish
Peer reviewedYes
NRC numberNRC-ACRD PDB059
Export citationExport as RIS
Report a correctionReport a correction
Record identifiere168e842-4aba-4349-8a5a-a8b6df57eff6
Record created2020-10-23
Record modified2020-10-23
Report a problem on this page
Date modified: